(121a) Microfluidics-Based Biocatalyst-Product Separation for Continuous Enzymatic Processes. a Case Study on ?-Lactam Antibiotics Manufacturing
- Conference: AIChE Annual Meeting
- Year: 2020
- Proceeding: 2020 Virtual AIChE Annual Meeting
- Group: Pharmaceutical Discovery, Development and Manufacturing Forum
- Time: Monday, November 16, 2020 - 8:00am-8:15am
In this contribution, we propose a 3D-printed microfluidics-based device for downstream separation of the biocatalyst from the crystalline antibiotic product. Microfluidics is a technique characterized by the precise manipulation of fluid dynamics at the microscale and has shown a great promise for industrial processing applications. Among microfluidic devices, inertial microfluidics has experienced massive growth due to their high throughput, automation, operational simplicity, and low cost . In such a device, particles with different sizes are separated by taking advantage of inherent inertial lift and drag forces. Inertial microfluidics can be employed by various channel shapes and structures; spiral channels are frequently used for the aim of high-throughput particle focusing by reduction of lateral migration positions and they can be an ideal candidate for this particular application. Here, we focus on the separation of high aspect ratio crystals from spherical biocatalyst particles. The efficiency of the separation for different product and catalyst sizes, and potentials/challenges for scaling up the proposed separation strategy will be discussed.
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