Immune-Modulating Scaffolds in Drug Delivery and Bone Tissue Engineering
- Conference: Translational Medicine and Bioengineering Conference
- Year: 2017
- Proceeding: 2nd Bioengineering & Translational Medicine Conference
- Group: Poster Submissions
- Time: Saturday, October 28, 2017 - 6:30pm-7:30pm
In our current model, we use Layer-by-Layer (LBL) assembly, which characterizes the assembly of oppositely-charged polymers on a substrate to bind the protein of interest, in this case, it is Hep-PLL NPs. Of particular interest here is its charge, biocompatibility, and biodegradability. The solvent, nanoparticle, and scaffold are chosen so that all are alternating in charge and therefore the LBL model will be successful. Prior to incorporation on the scaffold, solutions of the nanoparticles at different compositions have been carried out to determine stability. To determine the stability and size of the nanoparticles, the Malvern Zetasizer was used. This equipment measures the size and zeta potential, or surface charge of the nanoparticle, and certain levels of charge indicate a stable equilibrium. Once included on the scaffold, Scanning Electron Microscopy (SEM) can be used to image the surface of the scaffold and determine surface coverage. Thereafter, the cytokines can be added to the nanoparticles and delivered into cell cultures on the PLGA-scaffold. Inflammatory markers of each cytokine can determine the effects of the immobilized NP-cytokine drug delivery system in vitro.