Recent Developments in Downstream Bioprocessing: Multimodal Chromatography, Affinity Precipitation and Integrated Bioprocessing

This presentation will focus on recent developments in our lab in three areas of downstream bioprocessing; multimodal chromatography, affinity precipitation and integrated bioprocessing. Fundamental studies into the nature of selectivity in multimodal chromatography will first be presented. This work will include chromatographic studies with various protein and multimodal ligand libraries and novel predictive approaches including course grained protein binding maps and quantitative structure activity relationship (QSAR) models using new classes protein molecular descriptors. Work will then be presented on affinity precipitation using smart biopolymers for the simultaneous recovery and purification of biological products. Finally, some results are presented on the development of integrated biomanufacturing systems using both affinity peptide capture as well as an optimized series of multimodal chromatographic operations.