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Microbial Cell Factory for C5 Platform Chemicals: Aminovalerate and Glutarate

Authors: 
Kim, E. Y., Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, Daejeon, South Korea
Oh, Y. H., Research Center for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, Daejeon, South Korea

5-Aminovalerate (5AVA) is an important C5 platform chemical. Escherichia coli was metabolically engineered for the production of 5-aminovalerate (5AVA) and glutarate. When the recombinant E. coli WL3110 strain expressing the Pseudomonas putida davAB genes encoding delta-aminovaleramidase and lysine 2-monooxygenase, respectively, were cultured and produced 3.6 g/L of 5AVA by converting 7 g/L of l-lysine. When the davAB genes were introduced into recombinant E. coli strain XQ56 allowing enhanced l-lysine synthesis, 0.27 and 0.5 g/L of 5AVA were produced directly from glucose by batch and fed-batch cultures, respectively. Further conversion of 5AVA into glutarate demonstrated by expression of the P. putida gabTD genes encoding 5AVA aminotransferase and glutarate semialdehyde dehydrogenase. When recombinant E. coli WL3110 strain expressing the davAB and gabTD genes was cultured in a medium containing 10 g/L l-lysine and 10 g/L α-ketoglutarate, 1.7 g/L of glutarate was produced. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557).]