(95ab) Glucan Particles As Potential Carriers of Natural Flavonoids for Treatment of Idiopatic Inflammation Diseases
Lymphatic cells are mainly present in inflammation area in organism where they are responsible for progress of inflammation. Glucan particles (GPs) interact with lymphatic cellÂ´s receptors on their surface and after that Î²-1,3-glucans are phagocytosed by the lymphatic cells. Therefore, GPs represent an effective oral delivery system for transporting of biological active substances. Curcumin is an anti-inflammatory flavonoid, which occurs in plants of family Zingiberacaea. In our project, curcumin was delivered to an inflammation in organism using GPs. Loading of curcumin into GPs was enabled via precipitation of curcumin from organic solvents. Curcumin dissolved in organic solvents was added to the GPs and through pores penetrated into cavity of glucan shell. Next, the solvent was removed and the curcumin was precipitated in glucan microparticles. GPs with the flavonoid inside were observed using confocal microscopy and SEM (scanning electron microscopy). The achieved content of curcumin was successfully analyzed by other methods, which can not be mentioned directly due to article in preparation. Furthermore, the curcumin-loaded GPs were tested in vitro on macrophageâlike cells and in vivo on laboratory mice in order to evaluate their effectivity. The obtained results show that these composites are promising materials for biomedical applications such as efficient treatment of inflammatory diseases (e.g. idiopatic inflammatory bowel disease, rheumatoic arthritis).