(3b) Composite Hydrogel Microparticles for Drug Delivery: Microfluidic Preparation, Antibody Functionalisation and Interaction with Cells
Monodisperse microparticles with the required size were prepared by using microfluidic techniques. By implementing extractive gelation technique on the microfluidic chip, it was possible to produce highly monodisperse alginate microparticles in the size range of blood cells, which is important for the future intravenous application. Effects of several variables on the final size and morphology of microparticles were evaluated. The iron nanoparticles and liposomes were encapsulated. The stability of liposomes in the alginate microparticles was investigated and remotely triggered release experiments were conducted.
Due to high stability of final microparticles in a buffer solution, their surface modification was feasible. Different approaches to microparticles modification by IgG antibody were evaluated. The most appropriate technique was modification through chitosan previously attachted on the alginate microparticles. Lastly, experiments describing interactions of functionalised micropaticles with the cells were evaluated.