(17c) Stealth Nanoparticles for Tumor Targeting: in vivo and in vitro Characterization (Invited)
Fluorescently labeled (fluorescein isothiocyanate) silica nanoparticles with mean diameter of â170 nm and zeta potential between -15 and -20 mV exhibited great colloidal stability in buffers and physiological media when modified with pHPMA. The amount of pHPMA and IgG M75 on the nanoparticle surface was evaluated via amino acid analysis with resulting 3.6 w.% and 1.0 w.%, respectively. Further in vitro characterization employing flow cytometry and two different cell lines (HT-29: expresses CA IX; DLD-1: does not express CA IX; negative control) showed specific interaction of IgG M75-modified nanoparticles with HTâ29 cell line while no nonspecific interactions were detected. Finally, the in vivo study on Nu-Nu nude mice with tumors grown from HT-29 cell line revealed that nanoparticles bearing IgG M75 antibody can accumulate in the tumor in tenfold higher concentration and are retained considerably longer than nanoparticles without specific targeting. Furthermore, the nanoparticles were detected in the blood even after 60 minutes after the intravenous administration, which might be contributed to the stealth pHPMA coverage, providing the sufficient contact with the organism and thus increases the possibility to reach the target.