(89g) The Effect of Nucleoid Associated Proteins on DNA Supercoiling
AIChE Annual Meeting
Monday, November 11, 2019 - 9:45am to 10:00am
We use a combination of coarse-grained simulation of NAPs and DNA and theory to investigate the effect that these proteins have on DNA supercoiling behavior. These proteins are preferentially bound to a bent DNA site via the binding and unbinding energy landscape. Additionally, these proteins change the local equilibrium angle upon binding, creating localized kinks in the DNA strand. We are able to capture experimental observations with our coarse-grained model. We can take this information to inform a theory that accounts for the effect that proteins have on the supercoiled system, such as the extension behavior, bending energy, and excluded volume that are dependent on concentration and force.