(770a) Studying RNA Modulated Protein Liquid-Liquid Phase Separation Using Coarse-Grained Models
In this work we extend our coarse graining approach to incorporate RNA and determine how it modulates protein phase separation. We directly simulate the co-phase separation of both the disordered domain of DEAD-box helicase protein LAF-1 and polyA RNA condensed together in a slab geometry using standard molecular dynamics simulations which are conducted over a range of temperatures. RNA-protein interactions lead to formation of condensed phases containing both RNA and protein. The presence of RNA lowers the concentration of protein in the dense phase and RNA-protein interactions also change the dynamics of proteins inside the condensed phase.
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