(598d) Crosslinking of Ovalbumin Nanoparticles Affects Cellular and Humoral Immune Responses
Here, instead of decorating preexisting particles with peptide or protein antigens of interest, we have developed a new class of protein nanoparticles that are entirely made of the model protein antigen (protein antigen nanoparticles, PANPs).
We found that as the Youngâs modulus of PANPs increases, their interactions with immune cells can be tailored. In in vivo immunization studies that were conducted in a prime-boost regimen, PANPs with intermediate Youngâs modulus elicited 50-fold and 10-fold increase in serum IgG titers compared to soluble antigen. Bone marrow-derived dendritic cells (BMDCs) internalized more PANPs with lower elasticity. Overall, by tuning the elasticity of PANPs, we were able to alter the humoral and cellular responses in terms of their uptake by BMDCs, OT-1 CD8+ T cells proliferation and antibody production.