(56c) Assembly Dynamics of Bacterial Pore Forming Toxins on Lipid Membranes
AIChE Annual Meeting
2019 AIChE Annual Meeting
Engineering Sciences and Fundamentals
Biomolecules at Interfaces II
Monday, November 11, 2019 - 8:34am to 8:51am
Biomembranes, self-assembled from lipid molecules, act as a platform for the assembly of several 2D and 3D biological structures. We use bacterial pore forming toxins (PFTs) to understand biological assembly on lipid membranes. PFTs, a major class of bacterial toxins are released as water soluble monomers. Upon binding the bilayer membrane, they undergo structural rearrangements and oligomerize to form a stable transmembrane pore. The molecular mechanisms giving rise to selective pore formation on target eukaryotic membranes have been unclear. Using single molecule imaging, we dissect the assembly pathway of Cytolysin A, a representative Î±PFT and demonstrate how cholesterol is a key component for effective pore formation and a possible means for selective targeting of PFTs to their target cells.