(342e) Reaction Kinetic Modeling and Process Optimization of Multi-Enzyme Biocatalytic Cascade Syntheses
- Conference: AIChE Annual Meeting
- Year: 2019
- Proceeding: 2019 AIChE Annual Meeting
- Group: Pharmaceutical Discovery, Development and Manufacturing Forum
- Time: Tuesday, November 12, 2019 - 2:10pm-2:35pm
In this presentation, the challenges and successes of biocatalytic reaction engineering will be summarized with several case studies. Complex enzymatic mechanisms are examined, including for multi-substrate multi-enzyme cascades. Reaction kinetic models are built using DynoChem and are validated against experimental data. The regression of kinetic parameters in these models allows for the demonstration of a statistically robust process understanding; and in some cases, these models allow for useful process prediction and optimization. Often, the model-building process has proven to be useful for identifying gaps in process understanding, which is useful to inform further development and risk mitigation.
This discussion will also include a particular emphasis on the value of pairing PAT and benchtop automation capability with experimental design methodologies to maximize information content in these fast-paced and complex development projects.