(239e) Novel Enzymes and Separations in Biotechnology-Identifying a Process Tailored Extraction System-
AIChE Annual Meeting
Tuesday, November 12, 2019 - 9:20am to 9:40am
One promising and fast measure accounting for complex interactions of biological macromolecules is the second osmotic virial coefficient (B22) characterizing the interactions between the biological macromolecules in solution and the cross virial coefficient (B23) characterizing interactions of the biological macromolecules with the salts / impurities. Assisted by a thermodynamic modeling approach, identifying the interactions of the low molecular weight components in the extraction system, allows for a fast and reliable first estimate on an appropriate extraction system.
B22 as well as B23 data was measured using composition gradient multi angle light scattering (GC-MALS) for different proteins and salts in aqueous solution.
One additional advantage using this approach is the fact, that the partition coefficient can be estimated using a hybrid ShortCut Modell based on a minimal set of experimental data. The results will help to improve the development and optimization of ATPS as well as the TAPPIR Technology used for the design of an efficient downstream process.