(186r) Real-Time Evaluation of Cellular Behavior upon Exposure to Metallic Organic Frameworks
Herein we proposed to advance our understanding of MOFs toxicity using both real-time and single point cellular assays. For this, we considered lung epithelial cells and ZIF-8, a lab-synthesized MOF as model systems and electric cell impedance sensing and colorimetric assays that assessed enzymatic functionality before, during and after cellular exposure. The above in vitro analysis complemented by microscopical observations helped evaluate viability and metabolic activity of the exposed cells and showed that there is a dose dependent toxic effect with identified time frames of action and subsequent cellular changes in adhesion or behavior. To our knowledge, this is a first combinatorial strategy used for MOFâs toxicity determination. The derived understanding the MOFsâ mechanisms of toxicity is envisioned to aid in minimizing particle deleterious effects to potentially lead to safe-by-design strategies when such material development is considered.