(177t) Bioprocess Optimization Production By Saccharoplyspora Erythraea from Bench to Semi-Industrial Scale | AIChE

(177t) Bioprocess Optimization Production By Saccharoplyspora Erythraea from Bench to Semi-Industrial Scale

Authors 

Abd Malek, R. - Presenter, Universiti Teknologi Malaysia
Mohamud, M. A., nstitute of Bioproduct Development, Universiti Teknologi Malaysia, Johor Bahru
Ramli, S., Universiti Teknologi Malaysia
EL Enshasy, H., Genetic Engineering and Biotechnology Institute
Sukmawati, D., Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Kampus B, Pemuda Street No. 10
Hanapi, S. Z., IInstitute of Bioproduct Development, Universiti Teknologi Malaysia, Johor Bahru
An antibiotic is defined as an organic compound produced by a microorganism which is inhibitory or repressor to the growth and metabolism of other microorganisms in small concentrations. Since Sir Alexander Fleming discovered Penicillin as the first antibiotic in 1928, several other antibiotics were also described, only few have been approved for pharmaceutical application because the mode of action against foreign microorganisms by other antibiotics could be harmful to human beings. Recently erythromycin marked as one of the leading and successful product in antibiotic market over the other antibiotics. Application of bioprocessing technology has leads to production of erythromycin at high quality and it is cheap in price. Erythromycin is made from Saccharopolyspora erythraea (formerly known Streptomyces erythraeaus). In this research; a previously optimized medium was used for cultivation of S. erythraea. The medium components were screened using conventional method and further optimized by statistical method known as Response Surface Methodology (RSM), previously. The maximal erythromycin concentration and cell dry weight obtained in shake flasks after medium optimized were 412.5 mg L-1 and 4.9 g L-1, respectively. It was found that medium optimization lead to the increase of erythromycin and cell dry weight by 30.43% and 17.3%, respectively. The optimized medium was composed of (g L-1): glucose, 45; yeast extract, 8; sodium nitrate, 4; dipotasium hydrogen phosphate, 2.5; sodium chloride, 1.0; and magnesium sulphate 0.5. The suitability of this medium for antibiotic production was further studied in 16-L bioreactor using controlled and un-controlled pH conditions. For controlled pH bioreactor, the maximal erythromycin, and cell dry weight obtained were 567.5 mg L-1 and 5.65 g L-1,respectively. It was observed that the maximum erythromycin production in controlled pH bioreactor was higher than that cultivated in bioreactor under un-controlled pH condition by 12.9%. This value was also about 15.8% higher than those results obtained in shake flasks. On the other hand, cell dry weight was about 17.7 % higher when compared to cell produced in un-controlled bioreactor.