(175q) Evaluation of Commercial Media and Feed for Improved Mab Production

Fed batch cell culture process has been a well-established process in the biopharmaceutical industry for last few decades. An emerging trend has mainly focused on developing or refining high-titer cell culture processes to meet increasing market demands and reduce manufacturing costs. To improve the product titer and reduce the manufacturing cost, significant advances have been made in the media and feed supplements offered by commercial vendors. We evaluated several commercially available media and feeds for improved titer. Two media and six feeds were screened in a sequential manner in ambr15 micro-bioreactors. Spent media analysis was performed on the best media and feed pair and key limiting components were identified. Supplementation with limiting amino acids, the product titer was doubled compared with the platform process. The modified fed-batch process was further verified in bioreactors at bench scale and similar cell growth performance and product titer was demonstrated.