(175bd) Role of the Liver during Infection with Blood Stag-Malaria: Pathology and Gene Expression

Malaria is a dangerous disease spreads in several countries worldwide.The role of the liver in survival in blood-stage malaria is only partially understood. This study investigates the role of the liver in fighting blood-stage malaria. Microarray analysis for gene expression in the liver of mice infected with Plasmodium chabaudi infected erythrocytes was investigated during the peak of parasitemia. Also, apoptotic changes and the oxidative status were investigated. Some apoptotic genes (Bcl2, Bax and Caspase 3) and some cytokines genes (IL-1 β, IL-6, IFN-γ and TNF-α) were regulated in the infected liver. The hepatic antioxidant (Glutathion and Catalase) as well as the hepatic oxidative stress markers (malondialdehyde, nitric oxide and ROS) were altered during P. chabaudi infection. Liver macrophages were reorganized in the liver and spleen. This was evidenced through the redistribution of the mice inoculated fluorescent microspheres in spleen and liver. Our current work using herbal extracts and biosynthesized nanoparticles against induced liver injury induced by blood stage malaria proves the liver role against the parasite. The identified genes expression indicates that the liver has an effective role against P. chabaudi malaria.