(97c) Microfluidic Modulation of Neural Differentiation of 3D Stem Cell Aggregates
Results showed that continuous media exchange was detrimental to generation of progenitor motor neurons, potentially because necessary cell-secreted factors were continuously removed. In contrast, exchanging media at defined intervals increased generation of progenitor motor neurons in microfluidic cultures. Interestingly, we also observed asymmetric patterns of differentiation in a subset of cell aggregates: portions of aggregates better protected from flow were observed to have increased numbers of motor neuron progenitors. Together, our results provide insight into how microfluidic media exchange can modulate cell behavior and differentiation. This can inform design of microfluidic culture conditions in future studies of directed differentiation and tissue morphogenesis.