(712i) Development of Hydrogel Encapsulated Carbon Nanotube Based Biomonitoring System and Its Applications Toward the Detection of Riboflavin Administration

Authors: 
Bakh, N., Massachusetts Institute of Technology
Lee, M. A., Massachusetts Institute of Technology
Nguyen, F. T., Massachusetts Institute of Technology
Gong, X., Massachusetts Institute of Technology
Bisker, G., Massachusetts Institute of Technology
Strano, M., Massachusetts Institute of Technology
The development of carbon nanotube based sensors, where amphiphilic polymers on the nanotube surface screen the interaction between analytes and the nanotube in an effect known Corona Phase Molecular Recognition (CoPhMoRe), provides an opportunity for long term in vivo biomonitoring of analytes due to their near-infrared fluorescence, lack of photobleaching, and resistance to degradation. In this work, we develop a platform to track an intensity modulated sensor response to the administration of riboflavin in mice. A hydrogel encapsulation method allows for the diffusion of small molecules, such as riboflavin, to reach the corona of the nanotube sensors, while maintaining the sensors in a localized patch for repeated imaging. A wide field of view imaging setup allows for spatiotemporal imaging of the hydrogel patches. Furthermore, a reference sensor that is invariant to the analyte was developed and used to account for imaging artifacts from breathing and movement to facilitate the biomonitoring of riboflavin.