(538b) Mechanisms for Controlled Dynamics in Gold Nanoparticle-DNA Origami Templates
AIChE Annual Meeting
Wednesday, October 31, 2018 - 1:00pm to 1:15pm
Thus, we investigated fundamental interactions between NPs and DNA origami structures with the goal of understanding energy transfer and kinetics between these structures. In this study, we employed a DNA origami hinge decorated with AuNP. The hinge arms were modified at specific mirror locations with single stranded (ss)-DNA overhangs complementary to ss-DNAs bound to AuNPs. Thus, binding of AuNPs, closed the hinges. Initially, the free energy landscape for closed hinges with AuNPs in varying locations was evaluated using Transmission Electron Microscopy (TEM). To evaluate the kinetics of AuNP-hinge opening in response to heating, hinges were modified with Förster Resonance Energy Transfer (FRET) reporters that indicated NP binding and hinge closure, respectively. Kinetics was then evaluated using fluorescence spectroscopy. Localized actuation was induced by laser actuation of AuNPs, which dissipates the optical input in the form of local heat. We compared these results to those obtained via bulk heating method. Bulk heating decreased time scales for actuation, which were limited only by heat transfer. We are currently evaluating the kinetics of structures undergoing local, plasmonic heating. This research could provide a strategy for increasing actuation kinetics in DNA origami structures, paving the way for light-sensitive, higher-order composites for potential nano-photonic applications.