(447c) Abnormal Nuclear Morphologies in Cancer: Role of Chromatin Regulators
Here we screened a library of chromatin regulators to discover proteins that are required for maintenance of normal nuclear shapes. Nuclear shapes were quantified using an elliptical Fourier analysis and top candidates were selected for further mechanistic studies. Features in the nuclear lamina (such as holes, grooves, and folds) were identified by an automated texture analysis. A library of epigenetic pharmacological agents was also screened and common agents that affect nuclear shape and lamin structure, in both model breast epithelial cells and metastatic breast cancer, were identified. Our results identify key epigenetic genes that may be involved regulating nuclear morphology.