(321d) Detection of Beta Carotene and Lutein Using Electrochemical Impedance Spectroscopy
AIChE Annual Meeting
Tuesday, October 30, 2018 - 1:40pm to 2:00pm
Electrochemistry is a cost effective sensing paradigm while maintaining sensitivity . Electrochemical impedance spectroscopy (EIS), an electrochemical method that measures the surface impedance when an alternating current is applied, is known to be an extremely sensitive surface technique. We investigated the use of EIS to correlate carotenoid solution concentration to glassy carbon surface interactions. Nanomolar levels of Î²-carotene and lutein were successfully detected using EIS in a solution with only analyte and in the presence of each other. Uniquely reported here will be the sensitivity and selectivity of Î²-carotene in the presence of lutein and the lutein in the presence of Î²-carotene. Surface-bound Î²-cyclodextrin was used to increase carotenoid detection sensitivity. Î²-cyclodextrin was chosen because of its ability to create host-guest complexes with hydrophobic molecules such as Î²-carotene and lutein. The sensitivity of carotenoid detection using Î²-cyclodextrin will be reported compared to unmodified electrodes. In conclusion, the significant progress we have made over the past year (i.e. detecting carotenoids in the presence of competing molecules and cyclodextrin surface modifications) will be reported and discussed.
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