(216b) Statistical Modeling to Optimize Lignin Conversion to Polyhydroxybutyrate By Cupriavidus Necator

Bioplastic, such as Polyhydroxybutyrate (PHB), produced from lignin adds value to lignocellulose biorefineries. PHB converted from alkaline pretreated lignin by Cupriavidus necator DSM 545 is too low (0.5 g/L) to be economical. The objective of this study is to apply statistical modeling to improve PHB production. First, screening experiments were performed with Placket-Burman design to study the main effect of oxidative enzymes (laccase, aryl alchohol oxidase (AAO)), mediators (ABTS, HOBT), surfactant Tween-80, silica nanoparticle Aerosil R816 and DMSO on PHB production. Based on their magnitude of effect, these factors were ranked in decreasing order; Aerosil R816, ABTS, Tween 80, laccase, AAO, DMSO and HOBT. Second, interaction effects of these factors will be studied. The factors that have a significant effect will be used as inputs in a subsequent central composite design to obtain the optimized condition for PHB production. Lignin depolymerization will be characterized by gel permeation chromatography.