(216b) Statistical Modeling to Optimize Lignin Conversion to Polyhydroxybutyrate By Cupriavidus Necator

Authors: 
Li, M., University of Nebraska-Lincoln
Wilkins, M. R., University of Nebraska-Lincoln
Eskridge, K., University of Nebraska-Lincoln
Bioplastic, such as Polyhydroxybutyrate (PHB), produced from lignin adds value to lignocellulose biorefineries. PHB converted from alkaline pretreated lignin by Cupriavidus necator DSM 545 is too low (0.5 g/L) to be economical. The objective of this study is to apply statistical modeling to improve PHB production. First, screening experiments were performed with Placket-Burman design to study the main effect of oxidative enzymes (laccase, aryl alchohol oxidase (AAO)), mediators (ABTS, HOBT), surfactant Tween-80, silica nanoparticle Aerosil R816 and DMSO on PHB production. Based on their magnitude of effect, these factors were ranked in decreasing order; Aerosil R816, ABTS, Tween 80, laccase, AAO, DMSO and HOBT. Second, interaction effects of these factors will be studied. The factors that have a significant effect will be used as inputs in a subsequent central composite design to obtain the optimized condition for PHB production. Lignin depolymerization will be characterized by gel permeation chromatography.