(19a) A Model of Oxidation Injury and an Antoxidant Drug Delivery Rescue Strategy
AIChE Annual Meeting
2018
2018 AIChE Annual Meeting
Materials Engineering and Sciences Division
Biomaterials for in vitro Tissue Models and Improved Therapeutic Strategies
Sunday, October 28, 2018 - 3:30pm to 3:48pm
We have previously demonstrated a method to create microparticles with high loads of NAC that protected OPCs from oxidant-induced injury. We hypothesize that a controlled, zero-order release of NAC will protect OPCs from photoinitiated LAP, but moreover, from oxidants over a longer time period. Core-shell particles have previously been shown to tune drug release over longer time frames, and results in a linear release rate, a particular challenge for delivery of small, hydrophilic drugs like NAC from polymer-based particles. Preliminary confocal images suggest that we can fabricate core-shell microparticles using an empty poly(lactic acid) shell and TRITC-BSA-loaded poly(lactic-co-glycolic acid) (PLGA) core. 2-hour BSA release of core-localized particles was half that of shell-localized particles, indicating a reduced burst release. A linear fit of cumulative BSA release profiles for one week yielded R2 values of 0.74 and -0.71 for drug in core-localized and shell-localized particles, respectively, indicating a near-zero order BSA release profile for core-localized particles. Future work aims to encapsulate NAC into the core of core-shell particles and characterize their therapeutic affect on LAP-injured OPCs over time. NAC core shell particles will also be encapsulated in a physiologically relevant 3D hydrogel/OPC system to examine the particlesâ therapeutic affect for longer time frames.