(198e) Adipose Tissue Stem Cells Bioengineered in Nano-Biomimetic Col Scaffolds for Skin Tissue Engineering

Background: There is a motivation for tissue engineering, which is defined as promotion of repair or regeneration of injured or malfunctioned tissue or organ. In this way, tissue engineering (TE) is employed for helping the patient body and to produce materials which mimic the body‘s in vivo tissue conditions. In addition, TE strategies showed promising results for improving the quality of regenerative therapeutics. Three dimensional (3D) biodegradable and biomimetic porous scaffolds are ideal frame works for skin tissue engineering.

Methods: In this study hybrid constructs of 3D scaffolds were fabricated by freeze-drying method from combinations of the type I Col, and synthetic poly lactic acid (PLLA) or poly capro lactone (PCL). Four groups of 3D porous scaffolds were fabricated and characterized by HNMR, FT-IR, SEM including: PCL, PCL-Col, PCL-PLLA, PCL-PLLA-Col. Adipose tissue derived mesenchymal stem cells (AT-MSCs) were seeded in those scaffolds and the viability, proliferation and adhesion of them were investigated using MTT assay and scanning electron microscopy (SEM).

Results and Discussion: The results showed that scaffolds containing Col in particular PCL-PLLA-Col with pore sizes near to 400nm and sufficiently interconnected , have significant greater potential (p<0.01) for encouraging AT-MSCs adhesion and growth.

Conclusion: The PCL-PLLA provided a mechanically strong mesh support and the type I Col microsponges encouraged cell adhesion and tissue formation. The outcomes of this study could be applicable for preparing artificial skin constructs.