(190m) NANOG Restores the Myogenic Differentiation Potential of Senescent Myoblasts
The anti-aging effects of NANOG were accompanied by pluripotency effects of NANOG on myoblasts, in which expression of NANOG in differentiation phase could inhibit myotube formation, therefore removal of this factor was required before the start of differentiation.
Interestingly, the rejuvenating effects of NANOG on senescent myoblasts were stable for 2 weeks after removal of this factor, suggesting that NANOG might have imparted epigenetic changes. This rescue of myogenic differentiation was accompanied by reversal of cellular morphology and amelioration of aging hallmarks (i.e. trimethylation of H3K9, reduction of reactive oxygen species, and decrease in the DNA damage marker gH2AX).
In conclusion, these results shed light on the potential of NANOG to restore the myogenic differentiation potential of senescent myoblasts and to reverse the loss of muscle regeneration due to aging.