(188ce) A Multifunctional Versatile 3D Melanoma Model for Rapid Micro-Needle Based in Situ detection of Disease Specific Biomarkers
AIChE Annual Meeting
2018 AIChE Annual Meeting
Food, Pharmaceutical & Bioengineering Division
Poster Session: Bioengineering
Monday, October 29, 2018 - 3:30pm to 5:00pm
The aim of this work was to validate the S100 detection, a marker that is upregulated in melanoma, on a microporous polymer based 3D melanoma model using a novel immunodiagnostic microneedle device.
METHODS:3D polymer (PU) based microporous scaffolds (5x5x2.5mm3) were developed as previously described11 and the metastatic melanoma cell line A-375 was injected and cultured in those scaffolds for 5 weeks. Quantitative assessment of cell viability took place with the MTS metabolic assay and evaluation of cell distribution within the PU matrix was conducted with Scanning Electron Microscopy (SEM). Viable (live) cells were visualised in situ with confocal laser scanning microscopy (CLSM) of several sections of each scaffold. Furthermore, the detection of the S100 marker was carried out with PLA microneedles. The PLA microneedle device was produced, surface modified and coated with the S100 antibody as previously described, followed by the detection of the antigen via immunoassay analysis on the microneedle surface 12.
RESULTS:The 3D microporous scaffolds were able to support long term growth of the A-375 cells, with the majority of them being viable until the culture endpoint. Dense melanoma cell masses adhered to the scaffold pores and were distributed throughout the 3D matrix. Additionally spatial S100 detection was achieved via immunodiagnostic microneedle administration on the surface of the 3D scaffolds. The intense signal detection profile revealed that the antigen capture was highly specific, indicating the ability of the 3D polymeric model to validate the microneedle array.
DISCUSSION & CONCLUSIONS: Our findings indicate that this 3D polymer based microporous system is a promising tool for ex vivo modelling of metastatic melanoma. The scaffold properties such as porosity, stiffness and elasticity can be easily controlled and tuned, making this scaffolding system a promising candidate for modelling different types of skin (e.g. aged skin). Furthermore, to our knowledge, this is the first time that a 3D in vitro melanoma model is used for validation of biomarker detection with microneedles. Our findings suggest that this 3D microporous melanoma scaffold can be used as a low cost tool for validation/screening of novel cancer detection methods and/or kits, replacing and/or reducing animal testing.
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ACKNOWLEDGEMENTS: This work was supported by the Department of Chemical and Process Engineering of the University of Surrey as well as an Impact Acceleration Grant (IAA-KN9149C) of the University of Surrey, an IAA âEPSRC Grant (RN0281J) and the Royal Society.