(188b) Bioprocess of Crude Cell Extract Preparation for Bacterial Cell-Free Protein Synthesis System

For the past decades, the cell-free protein synthesis (CECF) system has developed for predominant protein production platform that takes place outside of the cell and served as a linkage of discrepancy between genetic information to those phenotypes. Due to its unique ex-vivo environment, the CFPS system also provides a new insight of understanding the cellular processes by controlling the system directly without viability issue of the cell. Meanwhile, the recent advance of synthetic biology has been accelerating to build up new biological systems which consist of the natural and user-defined biomolecules by reconstructing the complex cellular processes in living systems. By jumping on this bandwagon, the CFPS system has revitalized as a versatile synthetic biology research toolkit. Although the cell extract contains the most important bio-machinery for the system, however, we still do not have “magic” cell extract preparation process which shows same performance across laboratories as well as robustness in a variety of applications. Here I look into the upstream and downstream bioprocesses of crude extract preparation for the better understanding of each steps for democratization the complex process in compliance with growing demand for the cell-free synthetic biology research.