(188aw) Improved Heterologous Production of Salicylate 2-O-?-D-Glucoside Though E. coli metabolic System Modification

Metabolic engineering provides an opportunity to access to the small but complex molecule compounds with superior function to disease therapy but of low production and impurity through its original production mechanism. Escherichia coli (E.coli) has long been tested to be an effective heterologous host for foreign genes’ expression and widely applied in both academic and industrial fields. In this study, different from the normal biosynthesis pathway build-up in which multi plasmids bearing the specific genes are involved, we enhanced and simplified biosynthesis pathway by integrating all these genes into only one plasmid and optimizing hosts’ biosynthesis capability. The integrated synthesis system enables effective gene translation, homogeneous production microenvironment, and efficient material transportation which promise a highly-improved mechanism. Based on the study, we succeeded in boosting production of a plant-based anti-inflammatory agent salicylate 2-O-β-D-glucoside (SAG) to 2.7g/L.