(171a) Incorporation of Process Intensification Options in a Fed-Batch Cell Culture Manufacturing Platform

Several cell culture process intensification approaches were explored at AbbVie for increasing the productivity and processing speed of our protein biologics, with minimal impact on product quality profiles. Included in this are: the use of 10 mL cryovials to shorten the duration of seed train expansion, the use of perfusion technology to facilitate high cell densities at the seed bioreactor stage, and the use of perfusion and alternating tangential flow technology to facilitate increased protein yields at the production bioreactor stage. These approaches were demonstrated individually across all cell culture unit operations at laboratory and pilot scales. The work detailed in this poster provides a snapshot of the future of our biologics manufacturing capability as we continue to incorporate “pieces of the puzzle” of process intensification for our biologics pipeline.