Ammonium-Functionalized Carbon Nanotubes for Nucleic Acid Delivery in a Hypoxia-Induced Model of Acute Kidney Injury

Acute kidney injury (AKI) is a detrimental side effect of pharmaceutical treatment, particularly chemotherapy, and abdominal surgery. Ammonium-functionalized carbon nanotubes (fCNT) are a renal small interfering RNA (siRNA) delivery platform that have been used to prevent cisplatin-induced AKI in mice. To explore other causes of AKI, a precision-cut hypoxia-induced murine model of AKI is described here. RNA interference (RNAi) therapy using siRNA against Trp53 and Mep1b, two genes responsible for AKI progression, bound to fCNT (fCNT/siTrp53/Mep1b) decreases renal expression of pro-inflammatory cytokines in prophylactically treated mice. A robust hypoxia-induced model of AKI is developed and used to probe transcriptome changes and the therapeutic effect of fCNT/siTrp53/Mep1b.