(697d) Microfluidic ChIP-Seq Device for Rapid and Parallel Analysis of Histone Modifications
The microfluidic system consists of 4 parallel units, which are controlled using a bank of computer controlled solenoid valves and syringe pumps. Each run generates 4 ChIP DNA samples (which can be prepared into 4 sequencing libraries). The total on-chip operation takes as little as 1 h, with a hands-on working time of less than 20 min. We demonstrate the testing of chromatin samples from as few as 100 cells per assay and there was high reproducibility among 4 datasets generated by the same device giving Pearson correlation coefficients of 0.956, 0.970 and 0.870 for 1,000, 300, and 100 cell samples respectively.
The development of this automated ChIP assay lays the groundwork for large scale multiplexing which will allow fast, simultaneous analysis of multiple samples and marks, which will aid in clinical analysis of disease progression.