(676d) Fabricating Multi-Enzyme Catalyst in Reverse Emulsions
We then moved onto the fabrication of multi-enzyme micelle, using GOx and HRP as example. A proximity effect was observed, facilitating the intermediate consumption, as compared to both the individually encapsulated enzymes and their native counterparts in free solution. The proximity effect was further confirmed using catalase (CAT) to compete with the GOxâHRP microgel, in which inviable H2O2 was obtained by CAT, indicating that H2O2 produced by GOx was uptaken by HRP instead of entering the bulk solution. The GOx-HRP microgel remained 95% of the initial activity after 10 cycles. The circumvention of chemical modiï¬cation of the enzyme simpliï¬es the process, while the encapsulation yield may be further enhanced by molecular engineering of the phase forming polymer, making this method promising for multiple enzyme catalysis, particularly those in non-aqueous phase.
 Xiaoling Wu, Jun Ge,* Jingying Zhu, Yifei Zhang, You Yong and Zheng Liu*, Chem. Commun.,2015, 51, 9674