(583o) Biodegradation of Oligotrophic Waters Contaminated with Chloroacetanilides Using Bacterial Mesophylic Consortiums

Authors: 
Guzman Martinez, B., INSTITUTO POLITECNICO NACIONAL
Castro-Arellano, J. J., INSTITUTO POLITECNICO NACIONAL-ESIQIE
Rico, E., ESIQIE IPN
In this work, presents the biodegradation of chloroacetanilides: alachlor (2-chloro-N- (2,6-diethylphenyl) -N- (methoxymethyl) acetamide) and metolachlor (2-chloro-2 ', 6'- Diethyl-N-methoxymethylacetanilide); And chlorinated Carboxyamides; These herbicides are pre emergents and the most restricted in current legislation, and unfortunately the most widely used in the world. The environmental problem with chlorinated xenobiotics is that they accumulate both in soils and waters, specially in the lentic waters, and by their effects on health, being endocrine and mutagenic disruptors, They place them inside pollutants emergencies to be degraded. For this reason, there is a need to search for alternatives for degradation of these compounds among which according to the open literature: physicochemical and microbiological degradation processes were obtain. The research was carried out on a laboratory scale, using bacterial aggregates to treat contaminated oligotrophic lentic water (350 ppm) From the results found, possible mechanisms are proposed as degradation mechanisms used by these consortia and the mineralization of the chemical agent is verified. The selection of the bacterial consortia (between 60 via PCR RNA 16s), identification of the principal participating bacterial groups, were obtained and showed competitive biodegradation properties compared to those reported in the open literature in similar systems (Degradation above 95% at 10 days ),

Ecotoxicity tests were carry out under international standards (Vibrio fisherii) that validate the remediation achieved, results that will serve as a basis for the development of the solution of the problem raised.

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