(353a) Quantitative Analysis of the Deposited Nanoparticle Dose on Cell Cultures By Optical Absorption Spectroscopy
AIChE Annual Meeting
Topical Conference: Environmental Aspects, Applications, and Implications of Nanomaterials and Nanotechnology
Tuesday, October 31, 2017 - 12:30pm to 12:49pm
Nanoparticles made by aerosol-based technologies find a
wide range of promising applications (Pratsinis,
2010), though concerns for their potential health impact exist (Xia et al,
2009). Upon in vitro cytotoxicity evaluations of such particles, agglomeration
and settling need to be considered as they influence directly the particle dose
that is deposited on the employed cell cultures (Limbach
et al, 2005).
Here, nanoparticles of various chemical compositions and
sizes are made by flame spray pyrolysis (Madler et
al, 2002) and their in vitro cytotoxicity against murine macrophages is
evaluated (Sotiriou et al, 2014), accounting for their agglomeration and
settling in cell culture media. The agglomerate size distributions of the
particles in suspension are measured by dynamic light scattering (Wengeler et al, 2006) and their settling is evaluated by
ultraviolet-visible spectroscopy (Sotiriou and Pratsinis,
2010). The cell viability after 24 h incubation with such particle suspensions is monitored by a
tetrazolium salt reduction assay in both upright (Sotiriou et al, 2014) and
inverted cell culture configurations (Cho et al, 2011, Lee et al, 2014) (Figure
Figure 1. Relative viability of RAW 264.7 murine macrophages
after 24 h incubation with nanosilver or nanogold
suspensions as a function of nominal metal mass concentration in standard
upright and inverted cell culture configurations.
For given nominal particle mass concentrations, three
categories of nanoparticles can be distinguished, based on their cytotoxicity.
Materials such as gold induce no reduction in cell viability over a wide
concentration range (Sotiriou et al, 2014) and independent of the spatial
configuration of the cells (Figure 1). Materials such as silver particles of
relatively small crystal size (~8 nm) induce significant cytotoxicity in both
configurations due to silver ion release from their surface (Sotiriou et al,
2014) (Figure 1). Finally, materials such as agglomerated silica particles induce
higher cytotoxicity in upright than in inverted cell culture configuration, due
to enhanced particle-cell contact in the former.
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