(334f) Effects of Short-Term Magnetic Stimulation on MSCs Encapsulated in an Injectable, Magneto-Responsive Hydrogel
An injectable, magneto-responsive hydrogel was developed by chemically incorporating functional paramagnetic iron (III) oxide (Fe3O4) nanoparticles into a dual-gelling pNiPAAm-based hydrogel with degradable PAMAM-based crosslinking macromers. Rheological results showed that the nanoparticles played an active role as chemical crosslinkers[EA1]Â . Furthermore, the nanocomposite hydrogelâs physical and magnetic properties were determined utilizing differential scanning calorimetry, scanning electron microscopy (SEM), superconducting quantum interference device (SQUID) magnetometry, and tangential force measurements. Finally, the effects of short-term magnetic stimulation generated by an external magnetic field on encapsulated mesenchymal stem cells were evaluated through biochemical assays and gene expression. Together, these results demonstrated that this magneto-responsive hydrogel system holds promise as a non-invasive means to externally stimulate cell activity and promote regeneration in situ.
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