(302g) Effect of Combustion Particle Size on Pathologically Important Responses in Lung Cells
- Conference: AIChE Annual Meeting
- Year: 2017
- Proceeding: 2017 Annual Meeting
- Group: Topical Conference: Environmental Aspects, Applications, and Implications of Nanomaterials and Nanotechnology
- Time: Tuesday, October 31, 2017 - 9:40am-10:00am
The cdPM is generated from a premixed flat-flame burner combusting a jet-fuel surrogate at an equivalence ratio of 2.1. A nitrogen shroud and a glass housing minimize atmospheric effects. The sample is diluted, thermally denuded, and size separated using a differential mobility analyzer. Three different cdPM size fractions are examined (with particle diameters of 25, 45, and 65 nm) for their effect on oxidative potential, cell viability, cellular uptake, inflammatory response, cytochrome P450 (CYP) 1 A1 and 1 B1 mRNA induction, and the activation of transient receptor potential Ankyrin-1 (TRPA1). The biological outcomes are evaluated in A 549 human alveolar basal epithelial cells, with the exception of the TRPA1, which is evaluated in TRP over-expressing HEK293 cells. These results are a step towards understanding some of the interactions between understanding the links between combustion particle aging and health effects.