(302c) Evaluation of Biological Effects Using a Nano-Ceria Based Diesel Fuel Additive with in vitro Air-Liquid Interface Cell Exposure Systems of Different Flow Patterns
- Conference: AIChE Annual Meeting
- Year: 2017
- Proceeding: 2017 Annual Meeting
- Group: Topical Conference: Environmental Aspects, Applications, and Implications of Nanomaterials and Nanotechnology
- Time: Tuesday, October 31, 2017 - 8:20am-8:40am
The objective of this work is to determine the potential cytotoxicity and oxidative activity changes in lung cells cultured at the ALI after exposure to exhaust generated by a commercial, nano-cerium oxide based diesel fuel additive as directed with low-sulfur fuel. Two ALI cell exposure systems with two different flow patterns were utilized. Exhaust was generated by a partly loaded, single cylinder, four-stroke, air cooled, direct inject diesel power generator. The particle concentration was maintained at approximately 106 particles/cm3 as measured by a Scanning Mobility Particle Sizer. Deposition was measured gravimetrically on glass-fiber filters and blank cell culture inserts, qualitatively using Transmission Electron Microscopy, and online during exposure using quartz crystal microbalance measurements. These can be correlated to the biological effects of cytotoxicity and the oxidative activity, including reactive oxidative species generation and oxidative protection.
Acellular experiments suggest a correlation between the deposition and the type of fuel used for the newly designed Portable In Vitro Exposure Cassette. Using diesel exhaust, approximately one half of the filter mass is collected on the insert; whereas for additive fuel, approximately one sixth of the filter mass is collected. Preliminary cellular results suggest a decrease in cytotoxicity and no statistically significant effect on reactive oxygen species generation with the use of the nano-cerium oxide additive.