(200k) Peg Coated Fe3O4@MIL-100 (Fe) a Potential Carrier for Doxorubicin Delivery

PEG
coated Fe₃O₄@MIL-100 (Fe) a potential carrier for
doxorubicin delivery

Abhik
Bhattacharjee, Mihir Kumar Purkait and Sasidhar Gumma*

Department
of Chemical Engineering, Indian Institute of Technology Guwahati

*Email: s.gumma@iitg.ernet.in

In this present
study, Fe₃O₄@MIL-100 was explored as drug delivery
vehicle for anticancer
drug doxorubicin
hydrochloride.
First, Fe₃O₄
was prepared by co-precipitation method and has chosen for its low toxicity and
high magnetic saturation. MIL-100 (Fe), an iron based metal-organic frameworks
(MOF) was synthesized by solvothermal route with the help of organic linker
like trimesic acid (BTC) and Fe metal powder. A series of Fe₃O₄@MIL-100
carriers were prepared by varying the percentage mass of Fe₃O₄ in the raw precursor of MIL-100 at
temperature of 150 °C and characterized by X-ray diffraction, Nitrogen
adsorption-desorption isotherm and Transmission electron microscopy (TEM) methods.
Doxorubicin hydrochloride (DOX), a well-known anticancer drug for the treatment
of various cancer syndromes like leukaemia, lymphoma, and carcinoma was chosen
as a model drug for current study. The different drug loaded carriers were
prepared by mixing of DOX aqueous solution at a fixed concentration. The amount
of loaded DOX was estimated through analysis of the solution phase by UV-Vis
spectrophotometer at the wavelength of 480 nm. After that polyethylene glycol
(PEG) with molecular weight of 6000 and 9000 Da a polyether compound used in
medicinal applications was used to coat DOX loaded carriers i.e. DOX loaded
MIL-100 and Fe₃O₄@MIL-100. Typically, mass ratio of DOX
loaded carriers and PEG was maintained as 1:10. PEG with molecular weight of
6000 and 9000 Da were used for coating process. These PEG coated carriers were
further characterized by Field emission electron microscopy (FESEM) and
Thermogravimetric analysis (TGA).To carry out release experiments, these PEG
coated DOX loaded carriers, were dispersed into simulated phosphate-buffered
saline (PBS) solution of pH 7.4 at 37 °C in a shaking incubator. At a
predetermined time, the supernatants were collected for UV-Vis
spectrophotometer analysis. The amount of aliquots was taken from the mixer;
the same volume was replaced by fresh PBS. The release kinetics indicate about
sustained and stable release of DOX for several days by incorporating Fe₃O₄ in MIL-100 structure.

References:

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2.
P. Horcajada et.al. Nature, (2010), 9, 172.