(194w) Metabolic Engineering of Clostridium Aceticum for Acetone, Butanol and Ethanol Fermentation
AIChE Annual Meeting
2017
2017 Annual Meeting
Food, Pharmaceutical & Bioengineering Division
Poster Session: Food and Bioprocess Engineering
Monday, October 30, 2017 - 3:15pm to 4:45pm
In this study, we established methods for metabolic engineering of Clostridium aceticum DSM 1496 (ATCC 35044). Acetone and solvent production by C. aceticum will be studied individually and together. For acetone production, butyrate-acetoacetate CoA-transferase gene (ctfA/B) and acetoacetate decarboxylase (adc) gene from Clostridium acetobutylicum ATCC 824 will be introduced into C. aceticum. Also, ethanol and butanol production pathway was introduced by overexpressing aldehyde/alcohol dehydrogenase 2 (adhE2), thiolase (thl), butanol dehydrogenase (bdh) and BCS cluster (including crotonase (crt), putative butyryl-CoA dehydrogenase (BCD), electron-transfer flavoprotein (etf), and 3-hydroxybutyryl-CoA dehydrogenase (hbd) genes) from Clostridium acetobutylicum ATCC 824. Fermentation kinetics will be studied from sugar and CO2, as well as from CO2 and H2. This study aims at producing chemicals from CO2, H2 and sugar, which could help alleviate CO2 emission problem as well as increase fermentation yield.