(194i) Rapid and Facile Fabrication of Thermoplastic Organs-on-Chips
Methods:Â Here we present the facile, rapid, and scalable fabrication of a human intestine-on-a-chip using a commercial laser engraver systems, acrylic sheets, double sided adhesives, and a polycarbonate track etch membrane. The human intestine was recapitulated by culturing human Caco-2 cells in the apical fluidic compartment on a 1.0Âµm membrane while a syringe pump introduced cell culture medium into both the apical and basal fluid compartments. Caco-2 cells on chip were compared to control cultures of Caco-2 cells on commercial Transwell inserts via immunofluorescent staining for tight junction protein, F-actin, and cell nuclei. Mucus production on chip was compared to control Transwell cultures via immunofluorescent staining for mucin protein MUC2 and alcian blue staining.
Results:Â The fabrication methodology presented here boasts several key improvements compared to PDMS soft lithography: chip fabrication throughput was increased from days to hours while keeping material cost < $2 per chip, the technique doesnât require any specialized microfabrication, the thermoplastic chip construction enables oxygen tension control, and the double sided adhesives simultaneously act as fluidic compartments and provide a leak free bond without additional surface treatments like oxygen plasma or silanization. Immunofluorescent staining revealed the chip construction was biocompatible with human Caco-2 cells that similarly expressed tight junctions and F-actin compared to Transwell control cultures. Immunofluorescent staining and alcian blue staining showed that Caco-2 cells on chip produced more mucus compared to Transwell control cultures. We envision that the low cost, rapid, facile fabrication technique presented here will enable widespread access to micro physiological systems for researchers without microfabrication infrastructure or training.