(191bp) Purification of a Pegylated Protein
AIChE Annual Meeting
2017
2017 Annual Meeting
Food, Pharmaceutical & Bioengineering Division
Poster Session: Bioengineering
Monday, October 30, 2017 - 3:15pm to 4:45pm
PEGylation can bring along many challenges, one of which is the formation of process related impurities. Typically, size exclusion chromatography has been advantageous in removal of these impurities at small-scale, but this is not feasible for large-scale implementation. With the use of High Throughput Screens (HTS), several other purification methods were identified. This poster will highlight a challenging process in which large polymer-protein complexes were conjugated and subsequently purified downstream using HIC chromatography.