(167h) Dynamic Control of Gold Nanoparticle-Conjugated DNA Origami Templates
AIChE Annual Meeting
Sunday, October 29, 2017 - 5:15pm to 5:30pm
Our present study employs DNA origami hinges with single stranded DNA overhangs on the hinge arms as a model system. The sequence of these overhangs is complementary to that of single stranded (ss)-DNA on modified gold NP (AuNP) surfaces. A comparative study was conducted between the conformations of a closed hinge with varying locations of ss-DNA on the hinge arm in the presence and absence of AuNPs and analyzed by Transmission Electron microscopy (TEM) to determine the free energy landscape. Additionally, actuation kinetics of two different actuation pathways, strand displacement and bulk heating, were compared. Kinetics was studied in the bulk phase using fluorescence spectroscopy to analyze a Forster Resonance Energy Transfer (FRET) reporter system that indicated both NP binding and hinge closure. These results provided a comprehensive understanding of mechanical energy stored in NP-DNA origami composites. Preliminary experiments were performed for a third actuation scheme, localized actuation of composites using laser excitation to plasmonically heat AuNPs. Future experiments are investigating kinetics of structures undergoing local, plasmonic heating utilizing an in-house two source fluorometer instrument with separate excitation and modulation sources. This research could lead to higher order NP-DNA origami composites, that can store and release energy in response to light, with potential applications in nanophotonics.