(158c) The Effects of Filtration Condition on Virus Clearance
Virus filtration is conducted industrially in dead end mode. Membrane performance is shown to be affected by feed pH, ionic strength and feed buffer components. In this work, one of the FDA required model viruses, minute virus of mice (MVM) was spiked and used to investigate the effects of feed condition including pH, buffer and ionic strength on membrane performance and virus clearance using commercial virus filters in the presence and absence of model proteins. The interactions and possible associations among virus particles, model proteins and membrane materials modulated by the feed conditions were investigated. Their effects on membrane filtration performance and MVM clearance were elucidated.