Establishing a Co-Culture System for Clostridium Cellulovorans and Clostridium Aceticum for High Efficiency Biomass Transformation
AIChE Annual Meeting
Monday, November 14, 2016 - 10:00am to 12:30pm
C4 bio-refinery, such as butyrate and butanol production, is currently a research hotspot. However, undesirable waste products including H2 and CO2 result in lower product yield and a waste of energy. Clostridium aceticum has the ability to convert H2 and CO2 to acetate, which can be assimilated by other clostridia strains through the reversible acetate kinase and phosphotransacetylase pathway. This study setup a co-cultural system for C. aceticum and C. cellulovorans. Together they can convert the cellulosic biomass to butyrate or even butanol if a metabolic engineered strain is used, with considerably higher yields. Based on DSMZ 520 medium for C. cellulovorans and DSMZ 135 acetobacterium medium for C. aceticum, the practical pH ranges were evaluated for both strains. The pH 7.0-7.5 was suitable for both strains. By mixing these media with various ratios, an optimized formulation was defined for the co-cultural system. The evaluation of co-culture was conducted using glucose as substrate. The conversion rate of glucose to final products has increased by 5-10%.