The Effect of Calcium Chloride in Modified CHO Cell Growth for Antibody Expression

The antibody is a protein molecule produced from CHO-cells as a secreted molecule and it is further purified to ensure the product quality attribute for the patients. During manufacturing of the protein, it has been shown that cell growth is a key factor to obtain the appropriate protein concentration of the molecule. Some cell growth discrepancies have been associated to specific nutrients within the CHO-cells culture media. Previous data indicates that the concentration of CaCl₂ in the media may impact negatively CHO-cells growth. Therefore, this investigation was focused in measuring the growth of CHO-cells using cell culture media spiked with different CaCl₂ concentrations. The experiment was performed in shake flasks at constant temperature, CO₂ injection and base media content. The viable cell density, viability, pH, pO₂, pCO₂ and metabolites were measured for day 0 and day 3. After performed the experiment it was observed that the concentration CaCl₂ affect cell in terms of the viable cell density (VCD). Statistical analysis shows that for 0M of CaCl₂ less cell growth occurred, but for concentrations near 1M the cell growth was optimum.