(765d) Microfluidic Medip-Seq for Low-Input Epigenomic Analysis and Personalized Medicine
In this project, we developed an automated ultrasensitive microfluidic methylated DNA immunoprecipitation followed by next-generation sequencing (MeDIP-Seq) technology for profiling DNA methylomes. We extensively optimized design parameters for each and every step of MeDIP (e.g. sonication/crosslinking time, antibody concentration, washing conditions) in order to reach highest sensitivity of 0.1 ng DNA (or ~50-100 cells) as starting material for IP, which is roughly 4-5 orders of magnitude higher than the prevailing protocol and 2-3 orders of magnitude higher than the-state-of-the-art(~50 ng). With such sensitivity, we were able to study temporal dynamics in the DNA methylomes during the various stages of mammary cancer development from a transgenic mouse model.