(650f) Engineering Ultrastable Protein Scaffolds for the Controlled Assembly of Multifunctional Nanobiomaterials
We have recently reported the assembly of g-PFD monomers into geometrically defined templates of tunable dimensions and symmetry, as well as the controlled positioning of nanoparticles along the filaments. We aim to achieve more complex patterning, while expanding the applicability of g-PFD templates to diverse enzymatic systems. Fusing different enzymes to each subunit should enable periodic positioning of multiple enzymes along the filament to catalyze sequential reactions and metabolic pathways. In addition, we have demonstrated the ability to position cytochrome C proteins at high density along the filament as a first step to creating novel conductive nanowires. Finally, we are studying g-PFDâ??s ability to form 3D crosslinked multifunctional protein hydrogels. Ultimately, the ability to position functional molecules in a controlled manner will considerably enhance our ability to fabricate advanced multifunctional nanobiomaterials.