(650d) Chaperonin-Inspired Enzyme Protection By Mesoporous Silica
In vivo, enzymes are stabilised from high temperature or unfavourable solution conditions by chaperonins. Chaperonins, such as the GroEl/ES complex, help refold partially unfolded proteins within a narrow, cylindrical pore that becomes negatively charged. While chaperoninsâ?? complete mechanisms are complex, we are inspired by some of their fundamental properties when developing materials for enzyme immobilisation.
Mesoporous silica SBA-15 is used as a synthetic chaperonin analogue because of its controlled mesopore diameter and its negatively charged interior surface. Enzymes are immobilised on SBA-15 of different pore diameters, and their stabilities and biocatalytic activities are tested. SBA-15 is shown to be a valuable candidate for enzyme immobilisation, because it can protect enzymes from denaturing conditions.Â Selected findings include: immobilised myoglobinâ??s protection from acidic reaction conditions, with activity improvement of up to 350%; immobilised myoglobinâ??s complete protection from the protease pepsin; immobilised lysozymeâ??s protection from basic reaction conditions, with activity improvement of up to 200%; and the improvement of immobilised lysozymeâ??s activity by altering SBA-15â??s pore diameter.
More fundamentally, SBA-15 is a useful material for the systematic investigation of enzyme immobilisation parameters because of its highly tuneable pore diameter and its easily functionalised surface. The relative importance of hydropathy, electrostatic attraction, and steric confinement are studied in order to improve the future design of enzyme immobilisation materials.