(618h) Process Synthesis of Continuous Downstream Bioprocessing of Recombinant Intracellular Products
In this research, we explore the development of continuous downstream bioprocessing using process synthesis for recombinant intracellular products. The case study that we are conducting uses the extraction of beta-carotene from recombinant Saccharomyces cerevisiae. The separation and extraction pathway consists of five sections, 1) cell harvesting, 2) cell disruption, 3) cell extraction and recovery, 4) drying and tablet formulation and 5) recovery of media to be reused in the bioreactor. The superstructure is formulated as a mixed-integer nonlinear programming (MINLP) mode using profitability as the objective function based on that the cost of production for a 10% beta-carotene tablet found in literature3. The optimal topology for the downstream processing will be integrated with a continuous bioreactor model to give an optimal, continuous production implementation for intracellular product for comparison with traditional batch processes.
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